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easysep direct human ctc enrichment kit  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc easysep direct human ctc enrichment kit
    Easysep Direct Human Ctc Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+direct+human+ctc+enrichment+kit/pmc12046335-193-0-9?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    easysep direct human ctc enrichment kit - by Bioz Stars, 2026-08
    90/100 stars

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    easysep direct human ctc enrichment kit  (STEMCELL Technologies Inc)
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    STEMCELL Technologies Inc easysep direct human ctc enrichment kit
    Easysep Direct Human Ctc Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+direct+human+ctc+enrichment+kit/pmc12046335-193-0-9?v=STEMCELL+Technologies+Inc
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    easysep direct human ctc enrichment kit - by Bioz Stars, 2026-08
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    easysep™ direct human ctc enrichment kit  (STEMCELL Technologies Inc)
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    STEMCELL Technologies Inc easysep™ direct human ctc enrichment kit
    H1975 or H1975-GFP cells were labeled with CellTracker™ Green CMFDA dye, and a cell suspension of 1000 cells/mL was made. 100 µL of this fluorescently labeled H1975 cell suspension (containing approx. 100 cells) were added to both 5 mL of healthy donor blood and each control well (total 3). The spiked blood sample was incubated for 30 min before processing through the different CTC isolation systems: CellMag™, <t>EasySep™,</t> RosetteSep™, Parsortix® PR1 and PX+. B) In order to compare the recovery rate of in-cassette staining between the PR1 and PX+ systems, a similar protocol was followed using unlabeled H1975 cells. The enriched cells captured in the cassette were fixed and stained using the Parsortix® PR1 with Alexa Fluor (AF)-conjugated antibodies against Cytokeratins (CK 4,5,6,8,10,13, 18 and 19) and EpCAM (AF488, green), Vimentin (AF546, yellow), CD45 (AF647, red), and Hoechst nuclear stain (blue).
    Easysep™ Direct Human Ctc Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+direct+human+ctc+enrichment+kit/bio_rxiv__2024__02__05__578972-75-1-9?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    easysep™ direct human ctc enrichment kit - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier
    easysep tm direct human ctc enrichment kit  (STEMCELL Technologies Inc)
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    STEMCELL Technologies Inc easysep tm direct human ctc enrichment kit
    H1975 or H1975-GFP cells were labeled with CellTracker™ Green CMFDA dye, and a cell suspension of 1000 cells/mL was made. 100 µL of this fluorescently labeled H1975 cell suspension (containing approx. 100 cells) were added to both 5 mL of healthy donor blood and each control well (total 3). The spiked blood sample was incubated for 30 min before processing through the different CTC isolation systems: CellMag™, <t>EasySep™,</t> RosetteSep™, Parsortix® PR1 and PX+. B) In order to compare the recovery rate of in-cassette staining between the PR1 and PX+ systems, a similar protocol was followed using unlabeled H1975 cells. The enriched cells captured in the cassette were fixed and stained using the Parsortix® PR1 with Alexa Fluor (AF)-conjugated antibodies against Cytokeratins (CK 4,5,6,8,10,13, 18 and 19) and EpCAM (AF488, green), Vimentin (AF546, yellow), CD45 (AF647, red), and Hoechst nuclear stain (blue).
    Easysep Tm Direct Human Ctc Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+direct+human+ctc+enrichment+kit/pmc10530465-203-14-21?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    easysep tm direct human ctc enrichment kit - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

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    H1975 or H1975-GFP cells were labeled with CellTracker™ Green CMFDA dye, and a cell suspension of 1000 cells/mL was made. 100 µL of this fluorescently labeled H1975 cell suspension (containing approx. 100 cells) were added to both 5 mL of healthy donor blood and each control well (total 3). The spiked blood sample was incubated for 30 min before processing through the different CTC isolation systems: CellMag™, EasySep™, RosetteSep™, Parsortix® PR1 and PX+. B) In order to compare the recovery rate of in-cassette staining between the PR1 and PX+ systems, a similar protocol was followed using unlabeled H1975 cells. The enriched cells captured in the cassette were fixed and stained using the Parsortix® PR1 with Alexa Fluor (AF)-conjugated antibodies against Cytokeratins (CK 4,5,6,8,10,13, 18 and 19) and EpCAM (AF488, green), Vimentin (AF546, yellow), CD45 (AF647, red), and Hoechst nuclear stain (blue).

    Journal: bioRxiv

    Article Title: A comparative study of circulating tumor cell isolation and enumeration technologies in lung cancer

    doi: 10.1101/2024.02.05.578972

    Figure Lengend Snippet: H1975 or H1975-GFP cells were labeled with CellTracker™ Green CMFDA dye, and a cell suspension of 1000 cells/mL was made. 100 µL of this fluorescently labeled H1975 cell suspension (containing approx. 100 cells) were added to both 5 mL of healthy donor blood and each control well (total 3). The spiked blood sample was incubated for 30 min before processing through the different CTC isolation systems: CellMag™, EasySep™, RosetteSep™, Parsortix® PR1 and PX+. B) In order to compare the recovery rate of in-cassette staining between the PR1 and PX+ systems, a similar protocol was followed using unlabeled H1975 cells. The enriched cells captured in the cassette were fixed and stained using the Parsortix® PR1 with Alexa Fluor (AF)-conjugated antibodies against Cytokeratins (CK 4,5,6,8,10,13, 18 and 19) and EpCAM (AF488, green), Vimentin (AF546, yellow), CD45 (AF647, red), and Hoechst nuclear stain (blue).

    Article Snippet: The EasySep™ Direct Human CTC Enrichment Kit (Catalog #19657, StemCell Technologies) along with the “Big Easy” Magnet (Catalog #18001, StemCell Technologies) was used in this study to enrich cancer cells spiked into whole blood.

    Techniques: Labeling, Suspension, Incubation, Isolation, Staining

    After CTC isolation, the recovered cells from the CellMag™, EasySep™, RosetteSep™, PR1 and PX+ were incubated for 1 hour along with the associated control wells. Then, these wells were scanned using an imaging protocol (BioTek Lionheart FX automated microscope) to enumerate the total number of recovered cancer cells stained with Hoechst (DAPI channel) and CellTracker™ Green dye (GFP channel). B) The PR1 and PX+ stained cassettes were visualized using another automated imaging protocol. The percentage recovery rate was calculated by comparing the count of recovered cells against the average cell count from the control wells.

    Journal: bioRxiv

    Article Title: A comparative study of circulating tumor cell isolation and enumeration technologies in lung cancer

    doi: 10.1101/2024.02.05.578972

    Figure Lengend Snippet: After CTC isolation, the recovered cells from the CellMag™, EasySep™, RosetteSep™, PR1 and PX+ were incubated for 1 hour along with the associated control wells. Then, these wells were scanned using an imaging protocol (BioTek Lionheart FX automated microscope) to enumerate the total number of recovered cancer cells stained with Hoechst (DAPI channel) and CellTracker™ Green dye (GFP channel). B) The PR1 and PX+ stained cassettes were visualized using another automated imaging protocol. The percentage recovery rate was calculated by comparing the count of recovered cells against the average cell count from the control wells.

    Article Snippet: The EasySep™ Direct Human CTC Enrichment Kit (Catalog #19657, StemCell Technologies) along with the “Big Easy” Magnet (Catalog #18001, StemCell Technologies) was used in this study to enrich cancer cells spiked into whole blood.

    Techniques: Isolation, Incubation, Imaging, Microscopy, Staining, Cell Counting

    Approximately one hundred H1975 cells (pre-labeled with CellTracker™ Green) were spiked into 5 mL of whole blood and processed through the EasySep™, RosetteSep™, PR1 (harvest) and PX+ (harvest) methods. The enriched cell suspensions from each method were stained with Hoechst nuclear dye and the number of recovered cancer cells was counted to calculate recovery rate. Images were captured under 20X magnification using the BioTek Lionheart FX automated microscope and analyzed using ImageJ software. Scale bar represents 100 μm.

    Journal: bioRxiv

    Article Title: A comparative study of circulating tumor cell isolation and enumeration technologies in lung cancer

    doi: 10.1101/2024.02.05.578972

    Figure Lengend Snippet: Approximately one hundred H1975 cells (pre-labeled with CellTracker™ Green) were spiked into 5 mL of whole blood and processed through the EasySep™, RosetteSep™, PR1 (harvest) and PX+ (harvest) methods. The enriched cell suspensions from each method were stained with Hoechst nuclear dye and the number of recovered cancer cells was counted to calculate recovery rate. Images were captured under 20X magnification using the BioTek Lionheart FX automated microscope and analyzed using ImageJ software. Scale bar represents 100 μm.

    Article Snippet: The EasySep™ Direct Human CTC Enrichment Kit (Catalog #19657, StemCell Technologies) along with the “Big Easy” Magnet (Catalog #18001, StemCell Technologies) was used in this study to enrich cancer cells spiked into whole blood.

    Techniques: Labeling, Staining, Microscopy, Software